Device

Part:BBa_K4720021:Design

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


J23100+RBS+FleQ+TT+Pcl+Ppel+RBS+GFP+T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2553


Design Notes

We removed all the larger repeating sequences that we ourselves encountered problems with with recombination.


Source

This part is an adaptation of the part BBa_K4242013 by CUG China 2022.

References